Fig. 6

D.p. extracts have effects on MCs though the IgE-FcεRI axis. a SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. b MC degranulation by IgE cross-linkage, by c A23187, or d canine sera and extracts. Results were evaluated by one-way ANOVA with b Holm-Sidack, c Dunnett’s test, and d Student’s t-test. Boxes, the distribution of results (n = 3); solid and broken lines, the median and the mean. e Neurite outgrowth bioassay. A comparison with medium alone (Med) was performed by Student’s t-tests. Error bars, ± SEM (n = 3); n.s., no significance in difference; D.p,I, extract I; D.p.II, extract II