Fig. 5
D.p. extracts exhibit β-HEX activity. a β-HEX activity in the indicated concentrations of both extracts. An increase in OD values was statistically evaluated by one-way ANOVA with Dunnett’s tests. b Heat lability of β-HEX found in both extracts (50 μg/ml). The relative activity was calculated as ODwith heating/ODwithout heating and evaluated by two-way ANOVA with Holm-Sidack tests. c β-HEX activity in extracts (50 μg/ml) or rDer p 15 and 18 allergens (25 μg/ml). Significance in an increased OD was determined by Student’s t-tests. Error bars, ± SEM (n = 3); n.s., no significance in difference; D.p.I, extract I; D.p.II, extract II; Ctrl, buffer alone