Fig. 2

D.p. proteases are optimally active at elevated pH. a Casein and b gelatin zymography of extract I (D.p.I) digested at pH 5.0 or pH9.0. Intensity of arrow-indicated bands in the first lane (10 μg) was normalized by those detected at pH 8.0 (casein) or pH 7.6 (gelatin, both in white columns). Statistical evaluation was performed by one-way ANOVA with Tukey tests. Photos represent gray scale images of the typical data. Error bars, ± SEM (n = 3). The total protein load per gel lane: the lane 1, 10 μg; 2, 5 μg; 3, 2.5 μg; 4, 1 μg; 5, 0.5 μg