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Fig. 1 | World Allergy Organization Journal

Fig. 1

From: Innate function of house dust mite allergens: robust enzymatic degradation of extracellular matrix at elevated pH

Fig. 1

D.p. extracts exhibit proteolytic activity. a Casein and b gelatin zymography of extract I (D.p. I). Intensity of arrow-indicated bands was normalized by those detected in the first lane (10 μg, white columns) and evaluated by one-way analysis of variance (ANOVA) with Tukey tests. c Gelatin zymography of D.p.I using EDTA and Phen. Intensity of arrow-indicated bands in the first lane (10 μg) was normalized by those detected without chelators (white columns) and difference in data with or without chelators were compared by Student’s t-tests. Photos, gray scale images of the representative data. Error bars, ± SEM (n = 3). The total protein load per gel lane: the lane 1, 10 μg; 2, 5 μg; 3, 2.5 μg; 4, 1 μg; 5, 0.5 μg

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World Allergy Organization Journal

ISSN: 1939-4551

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