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Table 2 Allergen repertoire identified by LC-MS/MS analysis from the mix grass allergoid HP-SEC fractions. All fractions contained a group 1 allergen homologue.

From: Molecular fingerprinting of complex grass allergoids: size assessments reveal new insights in epitope repertoires and functional capacities

Grass species

Allergen

Accession number

Fraction 1 (2,249,594 Da)

Fraction 2 (973,404 Da)

Fraction 3 (424,493 Da)

Fraction 4 (113,736 Da)

   

Sequence

coverage

Peptide

number

Sequence

coverage

Peptide

number

Sequence

coverage

Peptides

number

Sequence

coverage

Peptide

number

Agrostis capillaris (Bent grass)

Agr a 1

gi|320606

-

-

-

-

46%

2

46%

2

Dac g 2

gi|1093120

-

-

-

-

17%

4

22%

5

Dactylis glomerata (Orchard grass/Cocksfoot)

Dac g 3

gi|14423759

14%

2

-

-

-

-

46%

4

Dac g 5

gi|14423122

-

-

-

-

31%

6

31%

8

Holecus lanatus (Velvet grass/Yorkshire fog)

Hol l 1

gi|3860384

-

-

-

-

28%

8

37%

9

Hol l 5

gi|2266625

5%

2

9%

2

21%

4

34%

8

Lolium perenne (Perennial ryegrass)

Lol p 1

P14946

12%

4

-

-

25%

7

30%

8

Lol p 2a

gi|126386

-

-

-

-

36%

4

57%

6

Lol p 3

P14948

14%

2

-

-

34%

4

34%

4

Lol p 4

gi|55859464

30%

11

30%

14

36%

16

37%

15

Lol p 5a

gi|332278195

4%

2

12%

3

24%

6

26%

5

Lol p 5b

gi|249 8 582

-

-

7%

2

12%

3

27%

7

Lol p 11

Q7M1X5

19%

3

23%

4

41%

13

13%

12

Phleum protense (Timothy grass)

Phl p 1

gi|1582250

15%

4

22%

6

20%

6

30%

8

Phl p 2

gi|1171009

-

-

-

-

-

-

19%

2

Phl p 3

gi|39841264

-

-

-

-

39%

3

39%

3

Phl p 4

gi|54144334

31%

14

32%

16

37%

19

23%

11

Phl p 5/5 a/5 b

gi|474100420

4%

2

-

-

24%

6

26%

7

Phl p 6

P43215

25%

2

-

-

45%

6

46%

7

Phl p 7

gi|14423846

-

-

-

-

29%

2

29%

2

Phlp p 11

gi|47606039

-

-

30%

5

51%

10

50%

8

Poa pratensis (Kentucky bluegrass)

Poa a 1

gi|320620

-

-

-

-

-

-

46%

2