Fig. 2

Degradation of Fel d 1 and CFE after PC-ions treatment. Concentrations of Fel d 1 (a) or Asp f 1 contained in CFE (b) in solution before and after sham or PC-ions treatment, as measured by ELISA, are represented as pre-treated, sham-treated and ion-treated, respectively. Concentrations of allergens are represented as means ± standard deviation. Significant differences at the level of **P < 0.01 was estimated by the post-hoc Student-Newman-Keuls test after non-repeated ANOVA. c Solutions of Fel d 1 before treatment and collected in the petri dish after sham or PC-ions treatment were loaded on a SDS-PAGE gel under reducing condition, which were labeled as pre-treated, sham-treated, and ion-treated, respectively. After visualizing bands by silver staining, the amino acid sequences of the main 15.0 and 6.5 kDa bands (chain 2 and chain 1, respectively) were analyzed by LC-MS and were confirmed to match the amino acid sequence of Fel d 1. Chain 1 and chain 2 for Fel d 1 were indicated with arrows. The lane labeled as Marker represents a molecular weight marker