Fig. 4
From: CD4+CD25highCD127- regulatory T-cells in COPD: smoke and drugs effect
CD4+CD25highCD127- regulatory T-cells after in vitro treatments. PBMCs from never-smoker healthy volunteers (n = 10), current smokers healthy volunteers (n = 10), former smoker COPD patients (n = 14) and current smoker COPD patients (n = 14) were cultured with/without drugs. Data are expressed as mean ± SEM and were normalized to the value of the untreated control. * = p < 0.05; ** = p < 0.01 versus NT. NT = cells treated with TGF-β + IL-2 alone; BUD1 = cells treated with TGF-β + IL-2 and budesonide 1 μM; BUD0.01 = cells treated with TGF-β + IL-2 and budesonide 0.01 μM; FOR30 = cells treated with TGF-β + IL-2 and formoterol 30 nM; FOR0.3 = cells treated with TGF-β + IL-2 and formoterol 0.3 nM; BUD1 + FOR30 = cells treated with TGF-β + IL-2 and budesonide 1 μM + formoterol 30nM; BUD0.01 + FOR0.3 = cells treated with TGF-β + IL-2 and budesonide 0.01 μM + formoterol 0.3 nM. NSHV: never-smoker healthy volunteers; CSHV: current smokers healthy volunteers; FSC: former smoker COPD patients; CSC: current smoker COPD patients; TGF-β1: transforming growth factor beta-1; IL: interleukin