Figure 1

Representative lumogallion staining of cryosectioned (6 μm section) THP-1 cells co-cultured with 50 μg/mL AlO(OH) Brenntag adjuvant for 24 h. Cell sections were incubated for 24 h in 100 μM lumogallion, 50 mM PIPES, pH 7.4. Slides were mounted with ProLong® Gold Antifade Reagent with DAPI. Lumogallion (orange) and DAPI-staining (blue) is depicted. The insert shows a close-up of an individual cell and white arrows highlight distinguishable adjuvant particles. Magnification X 1000. Kindly provided by Dr. Matthew Mold and Dr. Chris Exley, Birchall Centre, Keele, UK.