Figure 8

Notch signaling modulates cytokine production in response to pollen in allogeneic T cell stimulation assays. Dendritic cells were stimulated with different concentrations of aqueous birch pollen extracts (APE) or a Th2 differentiation cocktail and co-cultured for 4 days with allogeneic naïve CD4⁺ T cells in the absence or presence of the γ-secretase inhibitor DAPT. Cytokines IL-2, IL-4, IL-5, IL-10 and IFN-γ were measured in supernatants by ELISA. Shown are mean cytokine levels of 4 (Th2 controls) or 5 (APEs) independent experiments.