Figure 7

Aqueous birch pollen extracts reduce cytoplasmic expression of LPS-induced MyD88. Dendritic cells were stimulated for 24 h with medium, LPS (100 ng/ml), LPS + APE (10 mg/ml), LPS + APE < kDa (10 mg/ml) or LPS + APE > 3 kDa (10 mg/ml). Cytoplasmic lysates were subjected to SDS PAGE and Western blotting, and MyD88 was detected in the lysates by an anti-MyD88 antibody. A: Depicted is the result of one representative experiment. B: Quantitation of MyD88 relative to β-actin bands. Shown are results of 6 independent experiments. APE: aqueous pollen extract. *: p < 0.05, two-tailed Wilcoxon signed rank test.