Figure 4

Aqueous extracts from freshly collected birch pollen modulate the LPS-induced expression of notch ligands and MyD88. Aqueous extracts were prepared from three different freshly collected birch pollen specimens (birch trees #2, #10, #17). Relative mRNA expression of delta like-4, jagged-2 and MyD88 were determined by real-time PCR and expressed as 2^-ΔΔCT. 18S RNA served as house-keeping gene. Shown are means + SEM of 3 independent experiments.