Figure 3

Modulation of notch ligands and myD88 by aqueous birch pollen extracts is independent from pollen-derived of E ₁ -phytoprostanes. Monocyte-derived dendritic cells were incubated for 12 h with medium, LPS (100 ng/mL), LPS plus APE (10 mg/mL), LPS plus PGE₂ (10 μM) or LPS + PPE₁ (1 μM). Relative mRNA expression of delta like-1, delta like-4, jagged-2 and myD88 were determined by real-time PCR and expressed as 2^-ΔΔCT. 18S RNA served as house-keeping gene. Shown are the results of 5 (minimum) to 13 (maximum) independent experiments per condition. *: p < 0.05, two-tailed Wilcoxon signed rank test.