Figure 2

Reduction of LPS-induceddelta like-4mRNA expression by aqueous birch pollen extracts is dose-dependent and retained in a protein-free fraction. A: Monocyte-derived dendritic cells were incubated for 12 h with medium, LPS (100 ng/mL) or LPS plus different concentrations of total aqueous birch pollen extract. Shown are mean + SD of 3 independent experiments. B: Monocyte-derived dendritic cells were treated for 12 h with medium, LPS (100 ng/mL), LPS plus APE (10 mg/mL) or LPS plus protein-free APE (APE < 3 kDa). Shown are mean values + SD of 3 independent experiments. Relative expression of delta like-4 mRNA is expressed as 2^-ΔΔCT. 18S RNA served as housekeeping gene. APE: aqueous pollen extract.