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Figure 1 | World Allergy Organization Journal

Figure 1

From: Non-allergenic factors from pollen modulate T helper cell instructing notch ligands on dendritic cells

Figure 1

Regulation of notch ligandsdelta likeandjaggedby aqueous birch pollen extracts. Human monocyte-derived dendritic cells were incubated for 12 h in the presence of indicated stimulants (LPS: 100 ng/ml, APE: 10 mg/ml). A: Stimulation in the absence of LPS. B: Stimulation in the presence of LPS. Total RNA was extracted and cDNA subjected to qPCR using primers specific notch ligands delta like-1, −4, jagged-1 and jagged-2. Relative mRNA expression is given as 2-ΔΔCT with 18S RNA as house-keeping gene. Shown are the results of 4 (minimum) to 15 (maximum) independent experiments per condition. *: p < 0.05, two-tailed Wilcoxon signed rank test. APE: aqueous birch pollen extracts.

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