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World Allergy Organization Journal

Open Access

Positivity of contact dermatitis patch tests in an allergy and immunology dermatology service

  • Kleiser Aparecida Mendes1,
  • AssunÇao De Maria Gusmao Ferreira Castro1,
  • Claudia Soido Falcao Amaral1,
  • Maria Luiza Oliva Alonso1, 2,
  • Teresa Seiler1, 3,
  • Monica Ribeiro Oliveira1 and
  • Elizabeth Jorge Silva1
World Allergy Organization Journal20158:614

Published: 8 April 2015


Determine the prevalence of positivity to different groups of battery contact tests performed in a specialized Service.


300 patients were evaluated at 36 months, with a diagnosis of contact dermatitis. All of them underwent delayed skin test reading (patch test), with Brazilian Standard Battery of 30 substances. The test steps (placing two readings and grading of positive responses) were performed according to the standards established by the Brazilian Study Group of Contact Dermatitis (BSGCD) and the International Contact Dermatitis Research Group (ICDRG).


Of the 300 tests, positivity was observed in 194 (65%). 106 (35%) tests were negative and characterized contact dermatitis by primary irritant. There was a predominance of females (76%) compared to males (24%). The most affected locations were hands (palms and back - 64%), feet (back and plants - 41%), arms (31%) and face (26%). Positive results were obtained by groups of substances: Anthraquinone (0.5%), Balsam of Peru (5.1%), PPD mix (7.7%), Hydroquinone (2%), Potassium bichromate (19%) Propylene glycol (1.5%), p-tertiary Butyl Phenol (1%), Neomycin (4.1%), Irgasan (1.5%), Kathon CG (10.8%), Cobalt chloride (9.2 %), Lanolin (1.5%), Thiuram mix (3.6%), Ethylenediamine (3.6%) Perfume mix (6.7%), Mercaptobenzothiazole (mix) (2%), Benzocaine (3.6%) Quaternium 15 (1.5%), Quinoline mix (6.7%), Nitrofurazone (4.6%) Paraben mix (6.1%), Epoxy-resin (1.5%), Thimerosal (24. 2%) Turpentine (2%), Carba mix (7.2%), Promethazine (7.7%), Nickel sulfate (45.8%), Colophony (5.1%), Paraphenylenediamine (12.3%), Formaldehyde (6.7%).


Although positivity was observed in all groups, there was a predominance of Nickel sulfate, followed by Thimerosal and Potassium bichromate. This highlighted the importance of these substances in cases of allergic contact dermatitis admitted in our Service.

Authors’ Affiliations

Instituto De Dermatologia PROF Rubem David Azulay, Brazil
Hospital Universitário Clementino Fraga Filho Hucff-Ufrj, Brazil
Pontifícia Universidade Católica – RJ, Brazil


© Mendes et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.