Figure 5
From: M2 Muscarinic acetylcholine receptor modulates rat airway smooth muscle cell proliferation
Effect of selective muscarinic antagonists AF-DX-116 (M 2 AChR) and 4-DAMP (M 3 AChR) on ASMC proliferation at 5 and 10% FBS. A. Effect of increasing concentration of AF-DX-116 and 4-DAMP on the synergistic effect induced by Cch in presence of 5% FBS. ASMC (1× 103 cells/well) were cultured in 96 wells plates for 72 hrs. Cell proliferation was determined using a colorimetric method (MTS-PMS), measuring O.D at λ = 492 nm. (n = 5, for triplicate). The synergistic proliferation responses in the presence of 5% FBS and Cch (1 × 10-3 M) plus 5% FBS against basal condition were significant different (┼) p < 0.05. The muscarinic antagonists inhibitory responses were significant against the “0” Cch condition (*) p <0.05. The estimated Log IC50 ± SE were 4-DAMP + FBS = −7.34 ± 0.21; 4-DAMP + FBS + Cch = −7.38 ± 0.42; AFDX-116 + FBS = −7.26 ± 0.51 and AFDX-116 + FBS + Cch = −8.99 ± 0.45. B.- Effect of increasing concentrations of AF-DX-116 and 4-DAMP on the anti-proliferative effect induced by Cch (1x10-5 M), in presence of 10% FBS. ASMC (1 × 103 cells/well) were cultured in 96 wells plates for 72 hrs. Cell proliferation was determined using a colorimetric method (MTS-PMS), measuring O.D at λ = 492 nm. (n = 5, for triplicate). The muscarinic antagonists stimulatory proliferative responses were significant (*) p < 0.05 against the anti-proliferative Cch (1x10-5 M). The estimated Log IC50 ± SE were 4-DAMP = −7.11 ± 0.71 and AFDX- = −9.40 ± 0.37.