Figure 4

Rat ASMC proliferation in response to Cch and mitogens. ASMC (1 × 10³ cells/well) were cultured in 96 wells plates at 37°C/5% CO₂ with DMEM/F-12 medium (basal), Cch 10^-3 M, 5%FBS, EGF 5 and 10 ng/mL, and TNF-α 10 ng/mL during 24 hrs. Cell proliferation was determined using a colorimetric method (MTS-PMS), measuring O.D at λ = 492 nm. Data is the mean ± ES of n = 6 experiments for triplicate. Each mitogen proliferation responses vs basal was significant (*) p < 0.05. Moreover, significant differences (**) p < 0.05 between mitogen vs mitogen plus Cch condition were found.