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Figure 2 | World Allergy Organization Journal

Figure 2

From: M2 Muscarinic acetylcholine receptor modulates rat airway smooth muscle cell proliferation

Figure 2

Rat ASMC proliferation (A) and viability (B) in presence of mitogens and Cch. ASMC (2 × 105 cells/well) were cultured in 6 wells plates at 37°C/5%CO2 for 48 hrs in presence of DMEM/F-12 medium (basal), 10% FBS, EGF 10 ng/mL, TNF-α 10 ng/mL and Cch 10-5,-3 M. Cell proliferation and viability were determined using Trypan blue dye exclusion method. Data is the mean ± ES of n = 5 experiments for triplicate. The proliferation responses of each mitogen concentration vs. basal was significant (*) p < 0.05.

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