Figure 1From: M2 Muscarinic acetylcholine receptor modulates rat airway smooth muscle cell proliferationRat ASMC proliferation in response to FBS, EGF and TNF-α. ASMC (1 × 103 cells/well) were cultured in 96 wells plates at 37°C/5%CO2 for 24, 48 and 72 hrs in a medium without FBS (basal; ●), in presence of 5% FBS (∆), 10% FBS (▼), EGF 10 ng/mL (□), TNF-α 10 ng/mL (♦). Cell proliferation was determined using a colorimetric method (MTS-PMS), measuring Optical Density (O.D.) at λ = 492 nm. Data is the mean ± ES of n = 5 experiments for triplicate. The proliferation responses of each mitogen concentration vs. basal was significant (*) p < 0.05.Back to article page