Table 2 Studies comparing different techniques for specific IgE determinations
From: A WAO - ARIA - GA²LEN consensus document on molecular-based allergy diagnostics
Techniques compared | Allergens | Main findings | References |
|---|---|---|---|
ImmunoCAP & ISAC 50 | HDM, cat dander, birch, grass, and mugwort pollen | ROC curves demonstrated that CAP and ISAC performed equally well in cat, birch, and grass pollen. ISAC was slightly less sensitive in HDM and displayed a reduced sensitivity in mugwort pollen. | Wöhrl et al. [99] |
ImmunoCAP & ISAC prototype | Betula and grass allergens | Comparable sensitivity between CAP and ISAC. | Jahn-Schmid et al. [100] |
ImmunoCAP & ISAC 103 | grass and cypress pollen | Showed similar diagnostic performance. | Cabrera-Freitag et al. [101] |
ImmunoCAP & ISAC 103 | Multiples allergens | Concordance was 78.65% for positive results.Concordance was 93.57% for negative results. | Gadisseur et al. JACI [98] |
Reproducibility of ISAC 103 | rApi g 1, rBet v 2, nBos d 4, nGal d 1, nGal d 2, nGal d 3, rHev b 8, rPhl p 5, rPhl p 6, and rPhl p 7 | Excellent intra-slide, intra-assay, and inter-assay variability. rApi g 1, nGal d 3, and rPhl p 6 showed high variability in the individual analyses. | Cabrera-Freitag et al. [101] |
ImmunoCAP & ISAC 103 | Latex allergens | Similar performance | Ebo et al. [76] |
ImmunoCAP, ISAC 103, & ADVIA-CENTAUR | Pollen allergens | The 3 diagnostic methods were in agreement in 62.5% of cases. ISAC showed a deficiency in the detection of sensitivities to Salsola and Plantago; Advia-Centaur did not detect sensitizations to cypress. The concentration of sIgE in ISAC and ADVIA were significantly correlated for most pollen allergens. | Lizaso et al. [31] |
ImmunoCAP & ISAC 103 | 103 ISAC molecules | For low ISU values (0.3 to 1), the within-assay CV was very high (>100%), as expected; for medium (1 to <15) and high (15 or higher) ISU values, the CV was 17% and 8% respectively. The corresponding between-assay CVs were >100%, 33%, and 13.2%, respectively. | Melioli et al. [97] |
ImmunoCAP & ISAC 103 | Alt a 1 | Similar performance | Twaroch et al. [103] |