Figure 4
From: In Vitro Effects of Beta-2 Agonists on Skeletal Muscle Differentiation, Hypertrophy, and Atrophy
Beta-2 agonists effects on myosin heavy chains (MyHCs) protein expression. A, Western blotting analysis of MyHCs protein expression in differentiated myotubes. Upper panel is a representative immunoblot showing MyHC slow isoform expression; middle panel is a representative immunoblot showing MyHC fast isoform; α-tubulin (lower panel) was used as loading control. Lanes: 1, untreated cells; 2, clenbuterol-treated cells; 3, formoterol-treated cells; 4, salbutamol-treated cells; 5, salmeterol-treated cells. B, C, Graph of quantified data from Western Blot experiments with MyHC slow (B) and MyHC fast (C) isoforms. Histograms show the mean ± SD of 3 different Western blot quantifications.