Figure 1
From: In Vitro Effects of Beta-2 Agonists on Skeletal Muscle Differentiation, Hypertrophy, and Atrophy
ADRBs expression during cells differentiation. Time course of ADRB1 and ADRB2 mRNA expression during cell differentiation analyzed by qPCR. Cells were harvested for molecular analysis at 24 and 48 hours (proliferating myoblasts) and at 72 hours (D0, committed myoblasts) after seeding in GM and at 2 and 4 days (D2 and D4) after switch in DM. The results shown represent the mean of 3 independent experiments using independent cell cultures in each experimental setting. Bars symbolize qPCR data normalized versus ADRB1 mRNA expression value in untreated cells. ADRB2 were significantly more expressed than ADRB1 (P < 0.001). The significant increase of ADRB2 expression (P < 0.001) refers to the previous time point of culture (data normalized versus the baseline values). ***P < 0.001.