Figure 1

Total protein and IgE reactivity profiles of the grass pollen extracts. A, Proteins gold-stained or Western-blotted with (B) ATL IgE sera or (C) CBER IgE sera. Lane 1, molecular weight marker; lane 2, CBER grass standard; lane 3, in-house grass standard; lanes 4-16, single pollen extracts in the order listed in Table 1; lane 17, pollens extracted singly and pooled; lane 18, pollens extracted together.