Figure 2
From: Dexamethasone and Salbutamol Stimulate Human Lung Fibroblast Proliferation
The influence of dexamethasone and salbutamol on mast cell-induced fetal lung fibroblast proliferation. Fibroblasts were preincubated for 30 minutes with either dexamethasone (10-6-10-9 M) (A) or salbutamol (10-5-10-8 M) (B). Mast cell (HMC-1) sonicates (15 × 103 cells per well) were then added, and cultures were incubated for further 48 hours. In the control, the fibroblasts were incubated in DMEM 2% alone or with mast cell sonicates alone. Proliferation was evaluated as described in the legend to Figure 1. Data are the mean ± standard error of the mean of 5 experiments performed in triplicates. In medium alone, counts per minute of [3H]-Thymidine incorporation were 219 ± 3. **P < 0.01; ***P < 0.0001.