Figure 4
Pollen-and PCG-induced proliferation of lymph node cells. Cells from NC, pollen-sensitized, or PCG-sensitized rats were cultured with [3H]-thymidine in the presence of 10 μg of pollen (black histograms), 9 × 105 unwashed PCGs (gray histograms), and 9 × 105 washed PCGs (white histograms). CPM, thymidine incorporation by antigen-stimulated cells; CPM0, thymidine incorporation in control cells; NC, negative control group; pollen, rats sensitized and challenged with 0.5 mg of pollen; unw-PCGs, rats sensitized and challenged with 4.5 × 107 unwashed PCGs; w-PCGs, rats sensitized and challenged with 4.5 × 107 washed PCGs. All proliferative responses were significantly different from their respective NC value (P < 0.05), except for rats sensitized to unwashed PCGs and challenged with pollen grains.