Incrimination of Blomia tropicalisas a Potent Allergen in House Dust and its Role in Allergic Asthma in Kolkata Metropolis, India
© World Allergy Organization; licensee BioMed Central Ltd. 2010
Published: 7 May 2010
Theincreasing trend in allergic diseases has become obvious in the present day, especially in developing countries like India, because of many factors such as change in ambient air quality, increased air pollution, metamorphic change in living habits and lifestyle, and climate . Mites present in house dust represent a major source of allergens, resulting in different allergic manifestations all over the world, and hypersensitivity to these dust mites may play a pivotal role in pathogenesis of several allergic complaints including bronchial asthma. The present study evaluated the sensitization toward house dust and house dust mites among patients residing in Kolkata metropolis, India, who are suffering from allergic asthma.
The skin prick test was performed on a total of 1079 patients (585 males and 494 females) between the age group 5-50 years and 50 healthy controls using a variety of 16 common aero-allergenic extracts including 4 allergens of interest, viz. Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Blomia tropicalis and total house dust allergens. Total serum IgE level was measured by using the EIA technique and specific IgE levels against aforesaid allergens were detected with the Pharmacia ImmunoCAP 100 System. The influence of age and sex, if any, on allergen sensitivity was also investigated. All statistical analyses were performed using SPSS 10.0 for Windows and Zar .
The responses among patients with asthma to house dust and house dust mite allergen tests were as follows: house dust (96.22%), D. pteronyssinus (75.06%), B. tropicalis (72%), and D. farinae (63.72%). The frequency of positive skin response was found to be independent of age and sex. The total serum IgE levels in patients varied between 7.3 and 4040 IU/ml (mean 369 ± 26.51 IU/ml). Specific IgE antibody test proved that 83% patients showed sensitivity toward at least 1 of the allergens tested.
The results indicate that patients are highly sensitive to house dust and 3 other allergenic mites, namely, D. pteronyssinus, D. farinae, and B. tropicalis, as evidenced by the skin prick test, quantification of total serum IgE, and detection of allergen-specific IgE antibodies among patients of Kolkata. Although mites belonging to the genus Dermatophagoides have already been incriminated as a major source of allergen in house dust in India, this is the first time the role of B. tropicalis mites causing allergic asthma has been reported from an Indian population. Thus, the importance of B. tropicalis mite as an aetiopathological agent in causing various allergic manifestations among the Kolkata population should not be undermined and the allergen should be included in routine allergy testing.
House dust mites have been known to play an important role in the pathogenesis of bronchial asthma and other atopic diseases [1, 3, 4]. Mites are a very diverse and widespread group of animals that can be found in almost all habitats; however, in different parts of the world, they differ greatly in faunal makeup . During the 1960s, it was established that house dust mites belonging to the family Pyroglyphidae are the source of allergens that play a significant role in different allergic disorders including bronchial asthma [5–9]. The role of house dust mites belonging to the genus Dermatophagoides in asthmatic patients of Kolkata was already established by Saha  and Podder et al . Surprisingly, there is not a single published work from India incriminating the role of Blomia tropicalis mites in causing allergic disorders, though the sensitivity toward this mite species has been proven by several workers from different corners of the world [12, 13]. To provide patients with the best possible diagnosis and treatment, identification of offending allergen(s) is of prime importance. The present article deals with the incrimination of B. tropicalis, reported as important allergenic mites from elsewhere around the world, in the etiology of bronchial asthma among the Kolkata population, India.
Materials and methods
Selection of patients and control subjects
A total of 1079 patients clinically diagnosed as suffering from bronchial asthma in the age group 5-50 years were selected from the Outpatient Department of the Allergy & Asthma Research Centre and other private clinics in the Kolkata metropolitan area for this purpose. The personal and/or family history of individual patients were recorded in a well-prepared questionnaire including age of onset of symptoms, frequency of attacks, months of worst suffering, type of food intake, exposure to pet animals, influence of dusts, food, and nonspecific stimuli such as cold, exercise, and other irritant factors. Both male and female patients were categorized into 3 age groups ranging as follows: < 15 years (Gr. A), 16-40 years (Gr. B), and > 40 years (Gr. C). Cases with other organic and systematic diseases such as hypertension, diabetes, and also pregnant and breastfeeding woman were excluded. Selection of patients was done on the basis of the criteria mentioned earlier by Saha . A complete record of the patients' physical and clinical condition was also maintained.
Selection of control subject
Fifty healthy people (25 males and 25 females) belonging to the same age group as patients with no personal and/or family history of atopy and without any history of sensitivity toward dust inhalation were selected to serve as control subjects.
Stool examination for 3 consecutive days against individual patients and individual control subjects were done by flotation technique to exclude the possible interference of parasitic infections on the IgE level of patients' sera.
Allergy skin test to assess immediate hypersensitivity response
All patients and control subjects were subjected to conventional skin prick tests against 16 common aeroallergens including 4 distinct allergens, namely, total house dust and 3 species of house dust mite allergens, viz. D. pteronyssinus, D. farinae, and B. tropicalis. The mites were selected on the basis of their predominance in house dust of Kolkata metropolis.
Allergy skin prick testing was carried out by a conventional method of Gislason and Gislason  with the help of standard allergen extracts and working protocol provided by Credisol India Limited. The test was performed on the flexor side of the forearm of each patient following the method of Saha . Results were taken 20 minutes after the skin test and were interpreted following the criteria suggested by Grater et al . All antiallergic and steroid drugs were withdrawn from each patient 72 hours before the skin testing. Allergens giving 1+ to 4+ reactions were considered clinically significant.
Quantification of total IgE level
Measurement of total serum IgE was carried out by employing Enzyme Immuno Assay (EIA) using chemicals: Pathozyme Immunoglobulin (Ref: OD 417) supplied by Glaxo SmithKline Pharmaceuticals Ltd., Mumbai, India. The concentration of IgE is directly proportional to the color intensity of the test and the assay was calibrated against the World Health Organization  standard for IgE.
Detection of allergen-specific IgE antibodies
Detection of allergen-specific IgE antibodies in patients' sera against house dust (HD), D. pteronyssinus (DP), D. farinae (DF), and B. tropicalis (BT) was carried out by using the Pharmacia ImmunoCAP 100 System. The CAP was performed in collaboration with Pharmacia-authorized Metropolis Laboratory, Mumbai, India. The assay was calibrated against the World Health Organization Standard for IgE with a range of 0.35-100 KU/L for specific IgE. Specific IgE was measured against the house dust and 3 house dust mites (D. pteronyssinus, D. farinae, and B. tropicalis).
Paired Student t test with equal and unequal variances, Z-test, and contingent χ2 test were used to compare the allergic response toward different allergens among different age groups of patients and between sexes. Statistical analysis was performed using SPSS 10.0 for Windows and Zar .
Incidence of allergic response to house dust and house dust mites (D. pteronyssinus, D. farinae, and B. tropicalis)
Allergic Response to Common Inhalants (Pollens, Molds, and Others Including House Dust and Three Mites) in Bronchial Asthmatic Patients of Kolkata (n = 1079)
No. Positive Patients
Percentage of Positive Patients
Results of skin tests against total house dust and selected house dust mites (DP, DF, and BT) on 1079 patients revealed that 1035 (96.22%) patients showed sensitivity toward house dust. Because total house dust is a complex heterogeneous mixture of substances of both plant and animal origin, a high degree of sensitivity toward house dust is not uncommon . In the case of pyroglyphid mites the sensitivity was highest toward D. pteronyssinus (75.06%) followed by D. farinae (63.72%). Interestingly, the other mite B. tropicalis is responsible for causing allergies in 72% of the Kolkata population (Table 1).
Intensity of Skin Reaction Toward House Dust and Mites Among Asthmatic Patients of Kolkata
No. (%) Patients With Different Degrees of Skin Test Intensity
Total serum IgE levels among patients and control subjects
Total Serum IgE Level in Patients and Control Subjects Residing in Kolkata
Range of IgE IU/ml
Mean IgE IU/ml
Patients (n = 337)
369 ± 26.51
P < 0.05
Control (n = 50)
56 ± 34
Comparison of total serum IgE levels among different age groups
Total Serum IgE Levels in Different Age Groups of Patients
Age of Patients
Range of IgE (IU/ml)
Mean IgE (IU/ml)
< 15 years
415.47 ± 64.39
424.47 ± 65.88
> 40 years
315.64 ± 35.44
Statistical Analysis on Total Serum IgE Levels in Different Age Groups of Patients
Gr. A vs. Gr. B
P > 0.05
Gr. A vs. Gr. C
P > 0.05
Gr. B vs. Gr. C
P > 0.05
Comparison of total serum IgE levels among two sexes
Variation in Total Serum IgE Levels in Relation to Sex of Patients
Range of IgE (IU/ml)
Mean IgE (IU/ml)
n = 175 (51.92%)
383.74 ± 38.15
(df = 335)
P > 0.05
n = 162 (48.07%)
356.07 ± 36.73
Detection of allergen-specific IgE antibodies
Allergen-Specific IgE Reactivity Against HD, DP, DF, and BT Allergens by ImmunoCAP 100 System
No. and Percentage Sensitivity
Pharmacia ImmunoCAP sensitivity in relation to patients' age
Frequency of Positive Pharmacia ImmunoCAP Results Against HD, DP, DF, and BT in Patients of Different Age Groups
No. Positive Tests
3.7967 (df = 2)
P > 0.05
Statistical analysis (2 × 2 contingent χ2 test) indicated that the frequency of positive ImmunoCAP response to different allergens tested were not age dependent (P > 0.05), though the frequency of ImmunoCAP sensitivity in subjects aged 21 years and older was apparently higher.
ImmunoCAP response in relation to sex of patients
Prevalence of ImmunoCAP Sensitivity Against HD, DP, DF, and BT Allergens in Two Sexes
No. and Percentage of Positive Patients
P > 0.05
P > 0.05
P > 0.05
P > 0.05
At least 1
P > 0.05
A gradual increase in the incidence of bronchial asthma among the population of Kolkata during the last few years tempted us to identify the etiologic agents responsible for allergic complaints. The pilot screening test against 16 allergens of interest clearly showed that positive response to house dust and house dust mites were higher than the rest of the allergens tested. The skin prick test results against 4 selected aeroallergens of interest account for about 76% of allergic asthma among the Kolkata population. Skin test results on 1079 patients against HD, DP, DF, and BT showed that 96.22% patients were sensitive to HD, 75.06% to DP, 63.72% to DF, and 72% to BT allergens. These results confirm the earlier observation of Saha  who also reported the high degree of reactivity toward house dust and Dermatophaoides mites among patients with asthma from Kolkata. The present study also agrees quite well with other investigators  who also established the correlation between allergy and positive skin reaction. It seems that the frequency of allergic responses to different inhalants is the same in different age groups and for both sexes. However, these findings disagreed with Hannaway et al  who came to the conclusion that the rate of skin reactions was highly age dependent and peak reactivity occurred during adulthood with a sharp decline in the older age group. Murray et al  interpreted it as the natural decline in reactivity that occurs in older patients that may be attributed to either an actual decrease in immunologic reactivity or a decrease in the capacity of the skin to respond to immunologic challenge.
IgE was considered as the prime carrier of reaginic hypersensitivity in humans . According to Johansson  and Gleich et al,  the total serum IgE level is high in bronchial asthmatic patients. A wide range of serum IgE levels (7.3-4040 IU/ml) had been observed in this study group and difference of mean serum IgE levels between patients and control subjects was observed to be statistically significant in the present study. According to Ganju et al,  the basis of variations in IgE levels was polygenic with a possible effect of environmental and socioeconomic factors.
In the present study group it is to be noted that patients between the age of 16 and 40 years showed higher mean IgE levels in comparison to the other two groups, though the difference was not statistically significant. These findings are in conformity with the findings of Johansson  and Zetterstrom and Johansson,  who also reported that the serum IgE level did not depend on age. However, Khatua et al  suggested that the age of patients has a significant effect on the increase of total serum IgE level. However, the difference in mean serum IgE levels in male and female patients is not significant. This finding agrees with Zetterstrom and Johansson  and Batabyal et al . However, the present findings disagree with those of Wood and Oliver,  Sharma et al,  and Barbee et al  who had the opinion that total serum IgE levels were influenced by sex and age. In conclusion, it can be mentioned that the increase of total serum IgE levels is neither age nor sex dependent.
Although skin testing is an excellent screening test to evaluate immediate hypersensitivity reaction, allergen-specific IgE antibodies test is definite proof of IgE-mediated diseases . Hence, the determination of specific IgE provides important information regarding the therapy to be adopted.
Analysis of allergen-specific IgE antibodies against house dust, D. pteronyssinus, D. farinae, and B. tropicalis results in relation to patients' age and sex revealed that the frequency of sensitivity toward 4 selected allergens is neither age nor sex dependent (P > 0.05). This finding correlated well with the observation made by Chew et al,  Kerkhof et al,  and Simpson et al . The present findings revealed that about 76% of the patients in Kolkata are sensitive to house dust and 3 mite allergens tested. Among these positive patients, 93.5% have elevated serum IgE levels. This proves the atopic status of those patients. In the present study allergen-specific IgE antibodies were detected in the patients as follows: 79.77% to HD, 71.91% to DP, 88.76% to DF, and 90% to BT allergens. In the case of skin response 96.22% of the patients were sensitive to HD, 75.06% to DP, 63.72% to DF, and 72% to BT allergens. This difference in sensitivity against 4 allergens of interest by 2 diagnostic methods may be due to the appearance of false-positive or false-negative results by skin prick test whereas demonstration of specific IgE antibodies to a defined allergen is a definite proof of IgE-mediated disease .
Thus, the present study established the role of 3 allergenic mites, namely, DP, DF, and BT, in house dust allergy as evidenced by the skin prick test, total serum IgE, and detection of allergen-specific IgE antibodies among patients of Kolkata. This is the first time the role of B. tropicalis mite causing various allergic diseases including bronchial asthma has been reported from the Indian population. The study suggests that, in areas where B. tropicalis forms part of acarofauna, sensitization to this mite is an independent risk factor for asthma and as a matter of fact the role of B. tropicalis in causing bronchial disorders cannot be ruled out or underestimated. In conclusion, it can be said that sensitization to pyroglyphid and nonpyroglyphid mites is evident in the Kolkata population. These findings indicated that in vivo and/or in vitro testing for sensitization to these 3 species of mites should be considered in routine allergological diagnosis procedures.
The authors are grateful to the University Grants Commission, New Delhi, India, for providing financial assistance (sanction no. F3-132/2003/SR). Thanks are also due to H. Biswas and I. Roy for their technical assistance.
- Ciftci IH, Cetinkaya Z, Atambay M, Kiyildi N, Aycan OM, Daldal N: House dust mite fauna in western Anatolia, Turkey. Korean J Parasitol. 2006, 44 (3): 259-264. 10.3347/kjp.2006.44.3.259.View ArticlePubMedPubMed CentralGoogle Scholar
- Zar JH: Biostatistical Analysis. 1999, New Delhi, India: Pearson Education Singapore Pte. Ltd, 1-663. (Indian Branch)Google Scholar
- Sporik R, Chapman MD, Platts-Mills TA: House dust mite exposure as a cause of asthma. Clin Exp Allergy. 1992, 22: 897-906. 10.1111/j.1365-2222.1992.tb02062.x.View ArticlePubMedGoogle Scholar
- Casas R, Ferrándiz R, Wihl JA, Fernández B, Dreborg S: Biologic activity of Dermatophagoides siboney and Blomia tropicalis allergens in exposed and unexposed mite allergic individuals. Effect of patient selection on biologic standardization of mite extracts. Allergy. 1999, 54: 392-396. 10.1034/j.1398-9995.1999.00875.x.View ArticlePubMedGoogle Scholar
- Platts-Mills TA, Thomas WR, Aalberse RC, Vervloet D, Chapman MD: Dust mite allergen and asthma: report of a second International Workshop. J Allergy Clin Immunol. 1992, 89: 1046-1060. 10.1016/0091-6749(92)90228-T.View ArticlePubMedGoogle Scholar
- Voorhorst R, Spieksma-Boezeman MIA, Spieksma FThM: Is a mite (Dermatophagoides sp.) the producer of the house-dust allergen?. Allergy Asthma. 1964, 10: 329-334.Google Scholar
- Spieksma FT, Spieksma-Boezeman MI: The mite fauna of house dust with particular reference to the house-dust mite Dermatophagoides pteronyssinus (Trouessart, 1897) (Psoroptidae: Sarcoptiformes). Acarologia. 1967, 9: 226-241.PubMedGoogle Scholar
- Warner A, Boström S, Möller C, Kjellman NI: Mite fauna in the house and sensitive to house dust and storage mites. Allergy. 1999, 54: 681-690. 10.1034/j.1398-9995.1999.00850.x.View ArticlePubMedGoogle Scholar
- Eggleston PA, Bush RK, American Academy of Asthma, Allergy and Immunology: Environmental allergen avoidance: an overview. J Allergy Clin Immunol. 2001, 107 (3 Suppl): S403-S405.View ArticlePubMedGoogle Scholar
- Saha GK: House dust mite allergy in Calcutta, India: Evaluation by RAST. Ann Allergy. 1993, 70: 305-309.PubMedGoogle Scholar
- Podder S, Chowdhury I, Das A, Gupta SK, Saha GK: Immediate hypersensitivity to common inhalants: an investigation of nasobronchial allergy patients in Kolkata, India. Allergy Clin Immunol Int: J World Allergy Org. 2006, 18 (3): 114-119. 10.1027/0838-19188.8.131.52.View ArticleGoogle Scholar
- Van Bronswijk JEMH: Parasitic mites of Surinam: Mites and fungi associated with house floor dust. Ent Ber Amst. 1972, 32: 162-164.Google Scholar
- Chew FT, Zhang L, Ho TM, Lee BW: House dust mite fauna in tropical Singapore. Clin Exp Allergy. 1999, 29: 201-206. 10.1046/j.1365-2222.1999.00493.x.View ArticlePubMedGoogle Scholar
- Saha GK: House dust mite sensitivity among rural and urban asthmatics of West Bengal, India: a comparison. Aerobiologia. 1997, 13: 269-273. 10.1007/BF02694494.View ArticleGoogle Scholar
- Gislason D, Gislason T: IgE mediated allergy to Lepidoglyphus destructor in an urban population--an epidemiological study. Allergy. 1999, 54: 878-883. 10.1034/j.1398-9995.1999.00996.x.View ArticlePubMedGoogle Scholar
- Grater WC, Dockhorn R, Boggs P, Don RL: Report of the American College of Allergists Committee on standarisation of allergic extracts. Ann Allergy. 1982, 49: 49-54.PubMedGoogle Scholar
- WHO: Dust mite allergens and asthma: a worldwide problem. International Workshop Report. Bull World Health Org. 1988, 66: 769-780.Google Scholar
- Simpson A, Green R, Custovic A, Woodcock A, Arruda LK, Chapman MD: Skin test reactivity to natural and recombinant Blomia and Dermatophagoides spp. Allergens among mite allergic patients in the UK. Allergy. 2003, 58: 53-56. 10.1034/j.1398-9995.2003.23354.x.View ArticlePubMedGoogle Scholar
- Hannaway PJ, Hyde JS: Scratch and intradermal skin testing. A comparitive study in 250 atopic children. Ann Allergy. 1970, 28: 413-419.PubMedGoogle Scholar
- Murray AB, Simons FE, Ferguson AC, Freigang B, Gerrard J, Gold M: Skin testing for allergy in children. Can Med Assoc J. 1983, 129: 824-830.Google Scholar
- Ishizaka K, Ishizaka T, Hornbrook MM: Physicochemical properties of human reaginic antibody. IV: Presence of a unique Immunoglobulin as a carrier of reaginic activity. J Immunol. 1966, 97: 75-85.PubMedGoogle Scholar
- Johansson SG: IgE in allergic diseases. Proc R Soc Med. 1969, 62 (9): 975-976.PubMedPubMed CentralGoogle Scholar
- Gleich GJ, Averbeck AK, Swedlund HA: Measurement of IgE in normal and allergic serum by radioimmunoassay. J Lab Clin Med. 1971, 77: 690-698.PubMedGoogle Scholar
- Ganju A, Baram P, Lim DT: Serum IgE levels in healthy black American children. A case for environmental influences on IgE. Ann Allergy. 1979, 43: 271-274.PubMedGoogle Scholar
- Zetterström O, Johansson SG: The concentrations measured by PRIST in serum of healthy adults and in patients with respiratory allergy. Allergy. 1981, 36: 537-547. 10.1111/j.1398-9995.1981.tb01871.x.View ArticlePubMedGoogle Scholar
- Khatua SP, Khatua S, Batabyal SK, Bhowmik PK: Immunological study of bronchial asthma. Indian Pediatr. 1986, 23: 677-680.PubMedGoogle Scholar
- Batabyal SK, Ghosh JM, Kundu S, Chanda S: Studies on skin sensitizing tests and serum IgE levels in normal and other allergic disorders. The Antiseptic. 1986, 37-42.Google Scholar
- Wood CBS, Oliver J: Serum IgE in asthmatic children. Relation to age, sex, eczema and skin sensitivity. Int Arch Dis Child. 1972, 47: 890-896. 10.1136/adc.47.256.890.View ArticleGoogle Scholar
- Sharma S, Kathuria PC, Gupta CK, Nordling K, Ghosh B, Singh AB: Total serum Immunoglobulin-E level in a case-control study in asthmatic/allergic patients, their family members and healthy subjects from India. Clin Exp Allergy. 2006, 36 (8): 1019-1027. 10.1111/j.1365-2222.2006.02525.x.View ArticlePubMedGoogle Scholar
- Barbee RA, Brown WG, Kaltenborn W, Halonen M: Allergen skin-test reactivity in a community population sample: correlation with age, histamine skin reactions and serum immunoglobulin. E J Allergy Clin Immunol. 1981, 68 (1): 15-19. 10.1016/0091-6749(81)90117-2.View ArticleGoogle Scholar
- Kerkhof M, Dubois AE, Postma DS, Schouten JP, de Monchy JG: Role and interpretation of total serum IgE measurements in the diagnosis of allergic airway diseases in adults. Allergy. 2003, 58: 905-911. 10.1034/j.1398-9995.2003.00230.x.View ArticlePubMedGoogle Scholar
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