Volume 7 Supplement 1

2013 WAO Symposium on Immunotherapy and Biologics

Open Access

Poster 1007: Universal allergen particle generation for inhaled allergen challenges in the fraunhofer environmental challenge chamber (ECC)

  • Jens M Hohlfeld1,
  • Katrin Lueer1,
  • Heike Biller1,
  • Horst Windt1,
  • Philipp Badorrek1,
  • Meike Mueller1,
  • Holger Ziehr1,
  • Dietrich Haefner2,
  • Norbert Krug1 and
  • Wolfgang Koch1
World Allergy Organization Journal20147(Suppl 1):P4

DOI: 10.1186/1939-4551-7-S1-P4

Published: 3 February 2014

Background

Efficacy testing of immunotherapy in field studies is often hampered by variation of airborne allergens. Standardized allergen exposure in challenge chamber settings might overcome aforementioned limitations. However, airborne allergens such as house dust mite (HDM) or cat allergen are not available as standardized source material with distinct particle size. Therefore, we developed a method for universal allergen particle generation and conducted a pilot study to clinically validate this challenge paradigm for house dust mite.

Methods

House dust mite allergen extract (ALK lyophilised SQ503 Der p, ALK-Abello, Wedel, Germany) was diluted in an aqueous solution of 10% lactose. The solution was spray-dried at various liquid feed rates leading to allergen aerosols with different pre-defined concentrations but a constant mass mean aerodynamic particle diameter of 13.5 µm. Particle size is dependent on the solute (lactose) concentration and can thereby be adjusted accordingly. In a single-blind, five-way cross-over pilot study 18 subjects with allergic rhinitis and sensitization to HDM were allergen-exposed for 4 hours at either 250 SQE/m3, 500 SQE/m3, 1000 SQE/m3, or lactose alone (0 SQE/m3) seven days apart. The dose of 500 SQE/m3 was repeated to investigate reproducibility. Total nasal symptom score (TNSS), anterior rhinomanometry, nasal secretions, exhaled NO, FEV1, and adverse events were assessed prior to and during the exposures.

Results

Allergen exposure was safe and significantly elicited symptoms of AR compared to room air exposure with a mean total nasal symptom score (TNSS) of 3.6±2.0 (mean±SD) at the highest allergen concentration. Lactose alone did not change TNSS (0.7±0.6) compared to pre-challenge level. Repeated exposure to 500 SQE/m3 induced a TNSS which was not different between the two challenge sessions. Objective measures of nasal flow and nasal secretions were in line with clinical symptoms.

Conclusions

We conclude that this universal allergen particle generation is safe, specific, and reproducible and can therefore be used for efficacy testing of immunotherapy.

Authors’ Affiliations

(1)
Fraunhofer Institute
(2)
Allergopharma GmbH & Co KG

Copyright

© Hohlfeld et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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