Volume 6 Supplement 1
Allied health – 3008. Comparison of anti-allergic effect of pneumococcal conjugated vaccine and pneumococcal polysaccharide vaccine in a murine model of house dust mite allergic rhinitis
© Kim and Kim; licensee BioMed Central Ltd. 2013
Published: 23 April 2013
It was discovered that pneumococcal vaccine can reduce the allergic disease.The purpose of the present study is to evaluate the anti-allergic effect of pneumococcal conjugated vaccine and pneumococcal polysaccharide vaccine in a murine model of house dust mite allergic rhinitis. We also evaluated difference between effect of both vaccines.
Forty mice were divided into four groups: control, Der f, pneumococcal polysaccharide vaccine (PV) , and protein conjugate polysaccharide vaccine (PCV). Allergic rhinitis was induced in BALB/c mice by intraperitoneal sensitization and intranasal challenge with Dermatophagoides farinae (Der f). The allergic symptom after the final challenge was recorded. Interferon (IFN)-γ, Interleukin (IL)-13, and IL-10 levels in nasal lavage fluid (NALF), as well as serum Der f-specific IgE levels were measured. The number of eosinophils in lamina propria was evaluated. The levels of T-bet, GATA-3, and Foxp3 mRNA expression in splenic mononuclear cells were determined by real-time polymerase chain reaction. A comparison of the frequency of CD4+CD25+Foxp3+regulatory T cells in splenic mononuclear cells were made by flow cytometry.
The T-bet m RNA level was lower in the PV and PCV group than Der f group (p< 0.05). The IL-13, GATA-3 mRNA level and serum Der f-specific IgE and eosinophil were lower in the PV and PCV group than Der f group (p< 0.05). Foxp3 mRNA expression in the PV and PCV group was elevated compared to the Der f group (p< 0.05). In flow cytometry, the PV group (p < 0.05) and the PCV group (p < 0.05) had higher percentages of CD4+ CD25+Foxp3+ T cells than the Der f group. In the PV group, the percentage of these cells was higher than that in the PCV group. (p=0.00).
These results suggest that the pneumococcal polysaccharide vaccines will suppress the allergen-specific Th2 response and enhanced the induction of regulatory T cells in a model of allergic rhinitis. And the process to work regulatory T cell can be different between the PV and PCV group.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.