Figure 3

Rat ASMC proliferation response to muscarinic agonist Cch. ASMC (1 × 10³ cells/well) were cultured in 96 wells plates at 37°C/5% CO₂ with increasing concentration of muscarinic agonist Cch in medium without FBS during 24 (○), 48 (□), y 72 (∆), and 10% FBS, during 24 (●), 48 (■), y 72 (▲) hrs. Cell proliferation was determined using a colorimetric method (MTS-PMS), measuring O.D at λ = 492 nm. Data are the mean ± SE of n = 6 experiments for triplicate. Mitogen responses of 10% FBS vs. basal was significant (*) p < 0.05. The inhibitory effect of Cch was significant (┼) p < 0.05.