Figure 2

Determination of molecular size under physiologic conditions. Refractive index signal (bold lines) and MW for native (black) and modified (gray) rFel d 1 determined from online HPSEC light scattering. Determined MW values of the eluting fractions (native: V_ret = 5.6-7.3, 7.6-7.8, and 8.6-8.9 mL; modified: V_ret = 5.6-6.8, 7.2-7.4, and 8.0-8.3 mL) are shown in thin dashed lines.